, Pınar KAYGIN 2
, Sezen YILMAZ SARIALTIN 3
, Can YILMAZ 4
, Gülçin ŞİMŞEK 5
, Serpil OĞUZTÜZÜN 2
, Tülay ÇOBAN 3
, Abbas Ali HUSSEINI 6
, Yusuf IZCİ 7
2Kırıkkale University, Faculty of Science, Biology Department, Kırıkkale, Turkey
3Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey
4Van Yüzüncüyıl University, Faculty of Science, Department of Molecular Biology and Genetics,Van, Turkey
5University of Health Sciences, Kecioren Training and Research Hospital, Department of Pathology, Ankara, Turkey
6Istanbul Gelisim University, Life Science and Biomedical Engineering Application and Research Center, Istanbul, Turkey; Istanbul Gelişim University, Department of Biomedical device technology, Vocational School of Health Services, İstanbul, Turkey
7University of Health Sciences, Gulhane Training and Research Hospital, Department of Neurosurgery, Ankara, Turkey
Abstract
Objective: This study aims to delineate the expression profiles of glutathione S-transferase-Mu (GST-M) isozyme and tumor protein 53 (p53) in both healthy and tumorous brain tissue. Subsequently, the obtained results were compared with clinical features and lifestyle factors to elucidate any existing associations or correlations.
Materials and Methods: The medical records of 149 patients with primary or metastatic intracranial tumors were retrospectively analyzed. Expression levels of GST-M and p53 proteins were subsequently compared between healthy and tumorous brain tissues using immunohistochemical staining. Additionally, the associated clinical features and lifestyle factors were assessed.
Results: A significant difference in the expression levels of GST-M was observed between tumorous and healthy brain tissues, with a higher frequency in tumor tissues (p <0.0001). In contrast, robust p53 expression remained absent in both normal (97.3%) and tumor (78.5%) tissues, while a significantly greater abundance of such samples was still detected among the cancer patient groups (p <0.0001). However, expression levels were not associated with clinical features or lifestyle risk factors in this study. Moreover, postoperative survival was not influenced by GST-M and p53 expression.
Conclusion: The study highlights the higher expression of GST-M in brain tumor patients, suggesting a potential role of GST-M in brain tumorigenesis.