Objective: Gender dysphoria is a condition where an individual is discontent with their assigned gender. Karyotyping is a part of the transition period before hormone therapy to detect the biological gender for most professionals in many countries. Thus, we discussed the requirements for chromosomal analysis of individuals with gender dysphoria considering clinical and genetic findings.
Materials and Methods: Karyotype analyses were used to evaluate 67 unrelated individuals.
Results: Five individuals transitioned from male to female, and the rest transitioned from female to male. No chromosomal abnormalities and/or chromosomal rearrangements were observed, except for an individual who had mosaic Turner syndrome.
Conclusion: This study evaluates the results of chromosomal analysis of individuals with gender dysphoria. From the findings of this study and the literature, we suggest that chromosomal analysis is unnecessary unless evidence for another disorder of sex development is found alongside clinical, laboratory, and radiological findings.