2Department of Molecular Biology and Genetics, Artvin Çoruh University, Institute of Science, Artvin, Turkey; Department of Occupational Health and Safety, Artvin Çoruh University Faculty of Health Sciences, Artvin, Turkey
3Department of Molecular Biology and Genetics, Karadeniz Technical University Faculty of Science, Trabzon, Turkey
Abstract
Objective: Type 2 diabetes mellitus (T2 DM) represents a complex metabolic disease with genetic heterogeneity and increasing worldwide prevalence. One of the candidate genes associated with T2 DM and diabetic nephropathy (DN) is the plasminogen activator inhibitor-1 (PAI-1) gene. The present research aimed to reveal the polymorphism frequencies of the PAI-1 gene 4G/5G and to investigate the role of this polymorphism in T2 DM and DN development.
Materials and Methods: The genomic DNA of the 261 individuals was included in this study. The polymerase chain reaction (PCR) method with 4G and 5G allele-particular primers was used to identify the polymorphism of PAI-1 4G/5G. The PCR products were evaluated using a CCD camera after 2% agarose gel electrophoresis.
Results: Although the frequencies of the gene genotypes differed statistically significant between 80 patients and 51 patients with and without DN, respectively, and the control group, no statistically significant difference was detected between those with and without DN. The 5G/5G genotype was found to be significantly higher in the patient group.
Conclusion: The findings suggest that there is a significant correlation between variants of the PAI-1 gene and the risk for T2 DM formation.