2Department of Immunology, Erciyes University School of Medicine, Kayseri, Turkey
3Department of Hematology, Erciyes University School of Medicine, Kayseri, Turkey
4Flow Cytometry Unit, Erciyes University School of Medicine, Kayseri, Turkey
Abstract
Objective[|]Our aim was to evaluate the contribution of flow cytometry (FC) analysis in patients with a histopathological diagnosis of mantle cell lymphoma (MCL) and to develop a reliable scoring system.[¤]Materials and Methods[|]We assessed the results of FC analysis in peripheral blood or bone marrow samples of 36 patients diagnosed with MCL by histopathological evaluation. Multivariate analysis identified 5 variables associated with MCL: CD23 negativity, CD5/19 common positivity, CD20/22 common bright positivity, FMC7 positivity, and clonal sIg bright positivity. A 5-point scoring system was devised using these parameters.[¤]Results[|]Twenty-five patients (69.4%) scored 5 points, 8 (22.2%) scored 4 points, and 3 (8.4%) scored 3 points. The diagnosis of MCL was confirmed by FC immunophenotyping in 91.6% of the patients.[¤]Conclusion[|]Our results suggest that a quintet diagnostic scoring system, which is considered to be reliable in the diagnosis of MCL, could make a substantial contribution to diagnosis and should be more widely introduced in practice.[¤]