2Department of Pathology, Erciyes University, Kayseri, Türkiye
3Department of Biochemistry Faculty of Medicine, Erciyes University, Kayseri – Turkey
Abstract
Purpose: Determination of the sustained damage in brain and heart tissues of rats due to exposure to chlorine gas inhalation and investigation of the role of N-acetylcysteine over that damage.
Materials and Methods: A total of 50 rats were split into 5 groups all of which contained 10 rats. No treatment was applied to the Group 1. Group 2 and 3 consisted 6th hour control and N-acetylcysteine groups, respectively; whereas Group 4 and 5 were enrolled as the 24th hour control and N-acetylcysteine groups, respectively. Rats were subjected to inhalation of 200 ppm chlorine gas for 20 minutes. Following chlorine gas inhalation, intraperitoneal 40 mg/kg N-acetylcysteine was administered to N-acetylcysteine groups. Same dose of N-acetylcysteine was given again 3 hours later via the same route.
Results: While there was no damage in the brain tissue at the 6th hour (p>0.05), there was a damage at the 24th hour (p<0.01). No damage was determined in the heart tissue at 6th and 24th hours (p>0.05). Histologically, N-acetylcysteine was observed to repair the damage in brain tissue which occurred at the 24th hour (p<0.01).
Conclusion: Damage occurs in brain tissue at the 24th hour following chlorine gas inhalation. N-acetylcysteine can reduce that damage in experimental animal model.
2Department of Pathology, Erciyes University, Kayseri, Türkiye
3Department of Biochemistry Faculty of Medicine, Erciyes University, Kayseri – Turkey
Amaç: Bu çalışmada klor gazı solutulan ratların, beyin ve kalp dokusunda oluşan hasarı tespit etmek ve bu hasar üzerine N-asetilsistein'in etkinliğini araştırmak amaçlanmıştır.
Gereç ve Yöntemler: Elli rat 10'arlı 5 gruba ayrıldı. Grup 1'e hiçbir şey verilmedi. Grup 2 ve grup 3, sırası ile 6. saat kontrol ve N-asetilsistein grubu, grup 4 ve grup 5, sırası ile 24. saat kontrol ve N-asetilsistein grubu olarak alındılar. Ratlara 20 dakika süreyle 200 ppm klor gazı solutuldu. N-asetilsistein gruplarına klor gazı verildikten sonra intraperitoneal olarak 40 mg/kg N-asetilsistein uygulandı. Aynı doz N-asetilsistein 3 saat sonra aynı yol ile tekrar verildi.
Bulgular: Beyin dokusunda 6. saatte herhangi bir hasar tespit edilmez iken (p>0,05) 24. saatte hasar gözlendi (p<0,01). Kalp dokusunda 6. ve 24. saatte hasar tespit edilmedi (p>0,05). Histopatolojik olarak N-asetilsistein'in 24. saatte beyin dokusunda oluşan hasarı düzelttiği görüldü (p<0,01).
Sonuç: Klor gazı inhalasyonu sonrasında beyin dokusunda 24. saatte hasar ortaya çıkmaktadır. Oluşan hasarı deneysel hayvan modelinde N-asetilsistein azaltabilmektedir.