Objective[|]The present study was aimed to determine the distribution and antimicrobial susceptibility of strains isolated from urinary tract infections in our region.[¤]Materials and Methods[|]In this study, the distribution and antibiotic resistance profiles of microorganisms isolated from the urine cultures of urology outpatient clinic and ward patients between December 2011 and May 2013 were retrospectively evaluated.[¤]Results[|]The most commonly isolated microorganisms in outpatient clinic patients were; E. coli (71%), K. pneumoniae (8.8%), P. aeruginosa (6.3%), and the most commonly isolated microorganisms in hospitalized patients were E. coli (61.3%), P. aeruginosa (12.3%) and K. pneumoniae (5.8%). Amikacin, gentamicin, ceftazidime, cephalothin, ciprofloxacin and trimethoprimsulfamethoxazole resistance rates of E. coli strains and cefotaxime and cephalothin resistance rates of K. pneumoniae were higher in hospitalized patients than that in outpatient clinic patients (p<0.05). While E. coli resistance to ampicillin, amoxicillin-clavulanate, gentamicin, nitrofurantoin, cefotaxime, ceftazidime, cefuroxime, ciprofloxacin and trimethoprim-sulfamethoxazole was significantly higher among male patients who were admitted to the outpatient clinic, ceftazidime and trimethoprim- sulfamethoxazole resistance was significantly higher among hospitalized male patients, in comparison to that in the female patients (p<0.05).[¤]Conclusion[|]As antibiotic resistance rates vary across centres, it will be beneficial that each region perform surveillance studies to determine local antibiotic resistance rates for developing treatment protocols.[¤]