, Pınar Kaygın 2
, Sezen Yılmaz Sarıaltın 3
, Can Yılmaz 4
, Gülçin Şimşek 5
, Serpil Oğuztüzün 2
, Tülay Çoban 3
, Abbas Ali Husseini 6
, Yusuf Izci 7
2Kırıkkale University, Faculty of Science, Biology Department, Kırıkkale, Turkey
3Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey
4Van Yüzüncüyıl University, Faculty of Science, Department of Molecular Biology and Genetics,Van, Turkey
5University of Health Sciences, Kecioren Training and Research Hospital, Department of Pathology, Ankara, Turkey
6Istanbul Gelisim University, Life Science and Biomedical Engineering Application and Research Center, Istanbul, Turkey; Istanbul Gelişim University, Department of Biomedical device technology, Vocational School of Health Services, İstanbul, Turkey
7University of Health Sciences, Gulhane Training and Research Hospital, Department of Neurosurgery, Ankara, Turkey
Abstract
Objective: This study aims to explore the expression profiles of the glutathione S-transferase-Mu (GST-M) isozyme and tumor protein 53 (p53) in both healthy and tumorous brain tissues. The findings are compared with clinical features and lifestyle factors to identify potential associations or correlations.
Materials and Methods: We retrospectively analyzed the medical records of 149 patients diagnosed with primary or metastatic intracranial tumors. The expression levels of GST-M and p53 proteins were assessed in healthy and tumorous brain tissues using immunohistochemical staining. We also evaluated the associated clinical features and lifestyle factors.
Results: There was a significant difference in the expression levels of GST-M between tumorous and healthy brain tissues, with tumor tissues showing higher expression (p<0.0001). Conversely, robust p53 expression was absent in both normal (97.3%) and tumor (78.5%) tissues. Nevertheless, a significantly higher prevalence of samples with p53 expression was found in the tumor group (p<0.0001). No associations were found between expression levels and clinical features or lifestyle risk factors. Furthermore, GST-M and p53 expression did not impact postoperative survival rates.
Conclusion: The findings indicate an elevated expression of GST-M in brain tumor tissues, suggesting a potential role for GST-M in brain tumorigenesis.