The Role of Folic Acid in Rat Embryo Development in a Hypoxic Environment: An Experimental Study
1Kirsehir Ahi Evran University, Mucur Vocational School, Kirsehir, Türkiye
2Deptartment of Anatomy, Erciyes University Faculty of Medicine, Kayseri, Türkiye
3Department of Anatomy, Ankara Medipol University Faculty of Medicine, Ankara, Türkiye
4Department of Nursing, Nigde Omer Halisdemir University Zubeyde Hanım Faculty of Health Sciences, Niğde, Türkiye
5Department of Anatomy, Afyonkarahisar Health Science University Faculty of Medicine, Afyonkarahisar, Türkiye
J Clin Pract Res 2024; 46(3): 251-258 DOI: 10.14744/cpr.2024.64375
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Objective: Folic acid (FA) is a key antioxidant with substantial metabolic roles, and research has demonstrated its effectiveness in reducing congenital anatomical development disorders. This study explores the impact of folic acid on embryo development under hypoxia-induced conditions in embryo cultures.
Materials and Methods: Female Wistar albino rats, aged 4–10 months and weighing 150–250 grams, were utilized for this research. Embryos were extracted from the maternal womb on the 9.5th day of pregnancy. We established six groups, each consisting of 10 embryos: Control (C), Hypoxia (H), 1 mmol FA (1FA), 2 mmol FA (2FA), Hypoxia + 1 mmol FA (H1FA), and Hypoxia + 2 mmol FA (H2FA). Following a 48-hour culture period, the groups were assessed morphologically.
Results: When comparing the morphological parameters of the Control and Hypoxia groups, it was statistically demonstrated that the Control group completed its development, whereas the Hypoxia group exhibited insufficient development (p<0.05). There were statistically significant differences between the Hypoxia group and the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups (p<0.05). Both the Hypoxia + 1 mmol FA and Hypoxia + 2 mmol FA groups demonstrated better embryonic development compared to the Hypoxia group (p<0.05).
Conclusion: The study has established that FA has positive effects on embryos exposed to hypoxic conditions, which result in developmental delays.