Objective[|]Demodex spp., usually located on the human skin, including the face and eyelashes, is a mandatory ectoparasite. The aim of this study was to evaluate the prevalence and factors affecting Demodex spp. in patients with depression.[¤]Materials and Methods[|]In this study, 63 depressed patients and 63 healthy controls were evaluated. To collect samples for analysis, a drop of glue containing cyanoacrylate was put on a lamella and the lamella was pressed on the certain areas of the face (forehead, cheeks, nose, and chin) for about a minute. Then it was carefully removed and the density of Demodex spp. in a cm2 was counted under a microscope. For the diagnosis, the presence of five or more Demodex spp. in a cm2 was considered to be positive.[¤]Results[|]In 23.8% of depressed patients (n=15) and in 9.5% of the control group (n=6), Demodex spp. was detected in the facial area. The presence of Demodex spp. in the facial area of depressed patients was significantly higher compared to the control group. When CGI severity scores of patients and the relationship between the severity of depression and the presence of Demodex spp. were compared, no significant difference was detected.[¤]Conclusion[|]Depression may be a risk factor for the infection of Demodex parasites because of impaired immune system as well as reduction of self-care and hygiene of the person. For the itchy lesions on the face of depressed patients, Demodex spp. infestation should also be taken into consideration.[¤]